SYNMIKRO Research Center
The Höfer lab studies the epitranscriptomic mechanisms of gene regulation based on NAD-capped RNAs in bacteria. To identify novel and important connections between redox biology, gene expression and regulation we are combining cell biological, biochemical, structural, chemical and bioinformatic approaches. Our studies focus primarily on the model organism Escherichia coli, where we have identified NAD to be attached to a specific set of regulatory RNAs in bacteria in a cap-like manner, and to modulate the functions of these RNAs. To understand the biological significance of NAD-capped RNAs we are highly interested into proteins that are able to READ (decode), to re-WRITE (encode) or to ERASE (remove) NAD-RNAs. We have the vision that the NAD-cap serves as a molecular hub for proteins!
1. Höfer K, Li S, Abele F, Frindert J, Schlotthauer J, Grawenhoff J, Du J, Patel DJ, Jäschke A: Structure and function of the bacterial decapping enzyme NudC. Nature Chemical Biology, 12:730-4, 2016.
2. Höfer K, Jäschke A, Epitranscriptomics – RNA modifications in bacteria and archaea, Microbiology Spectrum, 6:3, 2018.
3. Abele, F*; Höfer, K*; Bernhard, P; Grawenhoff, J; Seidel, M; Krause, A; Kopf, S; Schröter, M; Jäschke, A. A Novel NAD-RNA Decapping Pathway Discovered by Synthetic Light-Up NAD-RNAs. Biomolecules, 10, 513, 2020.