The Flow Cytometry and Protein Diffusion & Interactions Unit is part of the Max Planck Department of Systems and Synthetic Microbiology, which is equipped with several fluorescence microscopes dedicated to automatic imaging and FRET applications.
The unit helps with the design and execution of protein interactions and protein diffusion experiments. The measurements are performed on a Nikon wide field fluorescence microscope customized for automatic acceptor photobleaching and ratiometric FRET measurements, and on a Zeiss LSM880 laser scanning confocal microscope dedicated to fluorescence correlation spectroscopy and fluorescence lifetime imaging.
Furthermore the unit is equipped with the BD Fortessa Flow Cytometer (Cell Analyzer) and with the BD FACSAria Fusion (Cell Sorter).
The BD Fortessa is routinely used for measuring fluorescence in bacterial samples. The instrument is equipped with 4 laser lines (445, 488, 514 and 561 nm) and 13 fluorescence channels. Laser lines were selected in order to maximize the excitation of fluorescence proteins such as CFP, GFP, YFP and mCherry. The cytometer is endowed with a high throughput sampler to efficiently perform automatic measurements of 96-well plates (as fast as 15 minutes for one plate).
The BD FACSAria Fusion Cell Sorter is equipped with 5 laser lines (355, 445/488, 561 and 640 nm) and 14 fluorescence channels allowing complex multi-color sorting. Both the BD Fortessa and FACSAria Fusion are highly sensitive gel-coupled cuvette instruments, this allows to identify and sort even populations with low-fluorescence.