Electron Microscopy

Electron Microscopy

Description

The core facility for Electron Microscopy is integrated as part of the Laboratory for Cell Biology of Prof. Dr. Uwe-G. Maier. For different kind of samples, various preparation methods and techniques can be offered and applied. Beside classical methods like negative staining of cell suspensions or purified proteins, which is a straight forward technique, some more complex procedures are also performed:

• Freeze-etching and freeze-fracturing for the illustration of cell surfaces (BALZER freeze-etching unit),
• Chemical fixation and resin embedding of cells,
• Cryo-fixation via plunge-freezing for preservation of cellular ultrastructure,
• High-pressure freezing of cells and tissues (Wohlwend HPF Compact 02),
• Ultrathin sectioning of resin embedded samples (Leica EM UC7rt, Reichert Ultracut-E),
• Immunogold localisation of proteins,
• Sample preparation for scanning electron microscopy (SEM),
• 3-D reconstruction via electron tomography,
• Post-processing of data sets.

Therefore, it should be possible to find the best fitting method for the specific research interest and finally investigate the samples in a 200 kV Transmission Electron Microscope from JEOL (JEM-2100) or the 80 kV Zeiss CEM902.
The core facility has been conducted by Dr. Andreas Klingl who changed to the LMU Munich in July 2014.

Order Form

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Literature

Naß B, Pöll U, Langer J, Kreuter L, Küper U, Flechsler J, Heimerl T, Rachel R, Huber H, Kletzin A, (2014), "Three Multiheme c-Type Cytochromes from the Hyperthermophilic Archaeon Ignicoccus hospitalis: Purification, Properties and Localization"; Microbiology

Klingl A, Flechsler J, Heimerl T, Rachel R, (2013), "Archaeal Cells", Encyclopedia of Life Sciences;

Rachel R, Meyer C, Klingl A, Gürster S, Heimerl T, Wasserburger N, Burghardt T, Küper U, Bellack A , Schopf S, Wirth R, Huber H, Wanner G, (2010), "Analysis of the Ultrastructure of Archaea by Electron Microscopy", Methods in Cell Biology, 96:47-69

Klingl A., Moissl-Eichinger C., Wanner G., Zweck J., Huber H., Thomm M. und Rachel R. (2011). Analysis of the surface proteins of Acidithiobacillus ferrooxidans strain SP5/1 and the new, pyrite-oxidizing Acidithiobacillus isolate HV2/2, and their possible involvement in pyrite oxidation. Archives of Microbiology 193: 867-882.

Hempel F., Bozarth A.S., Lindenkamp N., Klingl A., Zauner S., Linne U., Steinbüchel A. und Maier U.G. (2011). Microalgae as bioreactors for bioplastic production. Microbial Cell Factories 10: 81.

Gonzalez N.H., Felsner G., Klingl A., Schramm F.D., Maier U.-G und Bolte K. (2011). A single peroxisomal targeting signal mediates matrix protein import in diatoms. PLoS ONE 6 (9): e25316.

Rachel R., Meyer C., Klingl A., Gürster S., Heimerl T., Wasserburger N., Burghardt T., Kueper U., Bellack A., Schopf S., Wirth R., Huber H. und Wanner G. (2010). Analysis of the Ultrastructure of Archaea by Electron Microscopy. Methods in Cell Biology 96: 47-69.