Sinorhizobium meliloti 1021: Sm6kPCR

  • PCR fragments directed against protein-coding orfs of S. meliloti: 6046

  • 70mer oligonucleotides directed against protein-coding orfs of S. meliloti: 161

  • alien DNA fragments (Stratagene), can be used as spiking controls: 3

Each microarray contains 6210 triplicate spots in 48 grids of 22 rows and 18 columns. The 48 grids were arrayed in a 4x12 pattern of 4 metacolumns and 12 metarows.

 

Downloads:

Browse microarray probes using GenDB.

 

References:

  • Rüberg S, Tian ZX, Krol E, Linke B, Meyer F, Wang Y, Pühler A, Weidner S, Becker A (2003) Construction and validation of a DNA microarray for genome-wide expression profiling in Sinorhizobium meliloti. J Biotechnol 106: 255-268.
  • Becker A, Bergès H, Krol E, Bruand C, Rüberg S, Capela D, Lauber E, Meilhoc E, Ampe A, de Bruijn FJ, Fourment J, Francez-Charlot A, Kahn D, Küster H, Liebe C, Pühler A, Weidner S, Batut J (2004) Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Mol Plant-Microbe Interact 17:292-203.
  • Hoang HH, Becker A, González JE (2004) The LuxR homolog ExpR, in combination with the Sin quorum sensing system, plays a central role in Sinorhizobium meliloti gene expression. J Bacteriol 186: 5460-5472.
  • Chao T-C, Becker A, Buhrmester J, Pühler A, Weidner S (2004) The Sinorhizobium meliloti fur gene regulates in dependence of Mn(II) the transcription of the sitABCD operon encoding a metal type transporter. J Bacteriol 186: 3609-3620.
  • Puskás L, Nagy Z, Kelemen JZ, Rüberg S, Bodogai M, Becker A, Dusha I (2004) Wide-range transcriptional modulating effect of ntrR under microaerobiosis in Sinorhizobium meliloti. Mol Gen Genomics 272: 275-289.

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