Sinorhizobium meliloti 1021: Sm6kOligo

  • 70mer oligonucleotides directed against protein-coding orfs of S. meliloti: 6208

  • 70mer oligonucleotides directed against transgenes (gusA, lacZ, nptII, aacC1): 4

  • 70mer stringency control oligonucleotides (80 % identity): 2

  • alien 70mer oligonucleotides: 12

  • alien DNA fragments (Stratagene), can be used as spiking controls: 3

Each microarray contains 6.229 triplicate spots in 48 grids of 20 rows and 21 columns. The 48 grids were arrayed in a 4x12 pattern of 4 metacolumns and 12 metarows. Alien oligonucleotides and 12 "housekeeping" genes were arrayed in 13 additional replicates.

Oligonucleotides directed against the S. meliloti 1021 genome and the alien oligonucleotide controls were taken from the Sinorhizobium meliloti Array Ready Oligo Set Version 1.0 (Operon).

 

Downloads:

Browse microarray probes using GenDB.

 

References:

  • Krol E, Becker A (2004) Global transcriptional analysis of phosphate stress responses in Sinorhizobium meliloti strains 1021 and 2011. Mol Gen Genomics 272: 1-17.
  • Yao S-Y, Luo L, Har KJ, Becker A, Rüberg S, Yu G-Q, Zhu JB, Cheng H-P (2004) The Sinorhizobium meliloti ExoR and ExoS proteins regulate both succinoglycan and flagella production. J Bacteriol 186: 6042-6049.
  • Capela D, Carrere S, Batut J (2005) Transcriptome-Based Identification of the Sinorhizobium meliloti NodD1 Regulon. Appl Environ Microbiol 71: 4910-4913.
  • Chao TC, Buhrmester J, Hansmeier N, Puhler A, Weidner S (2005) Role of the regulatory gene rirA in the transcriptional response of Sinorhizobium meliloti to iron limitation. Appl Environ Microbiol 71: 5969-5982.
  • Capela D, Filipe C, Bobik C, Batut J, Bruand C (2006) Sinorhizobium meliloti differentiation during symbiosis with alfalfa: a transcriptomic dissection. Mol Plant Microbe Interact 19: 363-372.
  • Bobik C, Meilhoc E, Batut J (2006) FixJ: a major regulator of the oxygen limitation response and late symbiotic functions of Sinorhizobium meliloti. J Bacteriol 188: 4890-4902.
  • Domínguez-Ferreras A, Pérez-Arnedo R, Becker A, Olivares J, Soto MJ, Sanjuán J (2006) Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. J Bacteriol 188:7617-7625.
  • Sauviac L, Philippe H, Phok K, Bruand C (2007) An extracytoplasmic function sigma factor acts as a general stress response regulator in Sinorhizobium meliloti. J Bacteriol 189:4204-4216.
  • Stiens M, Becker A, Bekel T, Gödde V, Goesmann A, Niehaus K, Schneiker-Bekel S, Selbitschka W, Weidner S, Schlüter A, Pühler A (2008) Comparative genomic analysis and ultrafast pyrosequencing revealed remarkable differences between the Sinorhizobium meliloti genomes of the model strain Rm1021 and the field isolate SM11. J Biotechnol 136(1-2): 31-37.
  • Rossbach S, Mai DJ, Carter EL, Sauviac L, Capela D, Bruand C, de Bruijn FJ (2008) Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc. Appl Environ Microbiol 74:4218-4221
  • Hellweg C, Pühler A, Weidner S (2009) The time course of the transcriptomic response of Sinorhizobium meliloti 1021 following a shift to acidic pH. BMC Microbiol 9:37.

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