Sinorhizobium meliloti 1021: Sm14kOLI

  • 70mer oligonucleotides directed against protein-coding orfs of S. meliloti: 6208

  • 50mer to 70mer oligonucleotides directed against intergenic regions of S. meliloti: 8080

  • 70mer oligonucleotides directed against transgenes (gusA, lacZ, nptII, aacC1): 4

  • 70mer stringency control oligonucleotides (70 % to 90 % identity): 17

  • alien 70mer oligonucleotides: 6

  • alien DNA oligonucleotides (Stratagene), can be used as spiking controls: 5

  • spotting buffer and empty position controls

Each microarray contains 14976 duplicate spots (total spot number: 29952) in 48 grids of 26 rows and 24 columns. The 48 grids were arrayed in a 4x12 pattern of 4 metacolumns and 12 metarows.

Oligonucleotides directed against the S. meliloti 1021 coding regions and the alien oligonucleotide controls were taken from the Sinorhizobium meliloti Array Ready Oligo Set Version 1.0 (Operon).

Oligonucleotides directed agains intergenic regions were designed using the OligoDesigner (Daniel Paarmann, BRF, CeBiTec, Bielefeld University).

 

Downloads:

Browse microarray probes using GenDB.

 

References:

  • Bahlawane C, McIntosh M, Krol E, Becker A (2008) Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility. Mol Plant-Microbe Interact 21: 1498-1509.
  • Valverde C, Livny J, Schlüter J-P, Reinkensmeier J, Becker A, Parisi G (2008) Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011. BMC Genomics 9: 416.
  • Schlüter J-P, Reinkensmeier J, Daschkey S, Evguenieva-Hackenberg E, Janssen S, Jänicke S, Becker JD, Giegerich R, Becker A (2010) A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti. BMC Genomics: in press.

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