Peer Reviewed Publications

 

 2017

Robledo M, Peregrina A, Millán V, García-Tomsig NI, Torres-Quesada O, Mateos PF, Becker A, Jiménez-Zurdo JI A conserved a-proteobacterial small RNA contributes to osmoadaptation and symbiotic efficiency of rhizobia on legume roots. Environ Microbiol. 2017 Apr 12. doi: 10.1111/1462-2920.13757

Ampattu BJ, Hagmann L, Liang C, Dittrich M, Schlüter A, Blom J, Krol E, Goesmann A, Becker A, Dandekar T, Müller T, Schoen C (2017) Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence. BMC Genomics: DOI 10.1186/s12864-017-3616-7

Baumgardt K, Melior H, Madhugiri R, Thalmann S, Schikora A, McIntosh M, Becker A, Evguenieva-Hackenberg E (2017) RNase E and RNase J are needed for S-adenosylmethionine homeostasis in Sinorhizobium meliloti. Microbiology SGM: doi:

 

2016

Saramago M, Peregrina A, Robledo M, Matos RG, Hilker R, Serrania J, Becker A, Arraiano CM, Jiménez-Zurdo JI (2016) Sinorhizobium meliloti YbeY is an endoribonuclease with unprecedented catalytic features, acting as silencing enzyme in riboregulation. Nucleic Acids Research, 2016 1 doi: 10.1093/nar/gkw1234

Fan B, Li YL, Mariappan A, Becker A, Wu XQ, Borriss R (2016) New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42. Biol Open: pii: bio.021501.

Krol E, Klaner C, Gnau P, Kaever V, Essen LO, Becker A (2016) Cyclic mononucleotide- and Clr-dependent gene regulation in Sinorhizobium meliloti. Microbiology;  2016 (10):1840-1856. doi: 10.1099/mic.0.000356. Epub 2016 Aug 16

Döhlemann J, Brennecke M, Becker A (2016) Cloning-free genome engineering in Sinorhizobium meliloti advances applications of Cre/loxP site-specific recombination. J Biotechnol 233:160-70.

Lacanna E, Bigosch C, Kaever V, Boehm A, Becker A (2016) Evidence for Escherichia coli diguanylate cyclase DgcZ interlinking surface sensing and adhesion via multiple regulatory routes. J Bacteriol 198:2524-2535.

Babski J Haas KA, Näther-Schindler D, Pfeiffer F Förstner KU, Hammelmann M, Hilker R, Becker A, Sharma C, Marchfelder A, Soppa J (2016) Genome-wide identification of transcriptional start sites in the haloarchaeon Haloferax volcanii based on differential RNA-Seq (dRNA-Seq). BMC Genomics 17:629.

Frage B, Döhlemann J, Robledo M, Lucena D, Sobetzko P, Graumann PL, Becker A (2016) Spatiotemporal choreography of chromosome and megaplasmids in the Sinorhizobium meliloti cell cycle. Mol Microbiol 100:808-823.

Medeot DB, Rivero MR, Cendoya E, Contreras-Moreira B, Rossi FA, Fischer SE, Becker A, Jofré E (2016) Sinorhizobium meliloti low molecular weight phosphotyrosine phosphatase SMc02309 modifies activity of the UDP-glucose pyrophosphorylase ExoN involved in succinoglycan biosynthesis. Microbiology (2016), 162, 552-563.

Schäper S, Krol E, Skotnicka D, Kaever V, Hilker R, Søgaard-Andersen L, Becker A (2016) Cyclic di-GMP regulates multiple cellular functions in the symbiotic α-proteobacterium Sinorhizobium meliloti. J Bacteriol: 198:521-535.

Robledo M, Jiménez-Zurdo JI, Becker A (2016) Antisense transcription of symbiotic genes in Sinorhizobium meliloti. Symbiosis 67: 55

 

2015

Robledo M, Frage B, Wright PR, Becker A (2015) A stress-induced small RNA modulates alpha-rhizobial cell cycle progression. PLoS Genet 11(4): e1005153.

Maier LK, Benz J, Fischer S, Alstetter M, Jaschinski K, Hilker R, Becker A, Allers T, Soppa J, Marchfelder A (2015) Deletion of the Sm1 encoding motif in the lsm gene result in distinct changes in the transcriptome and enhanced swarming activity of Haloferax cells. Biochimie. 117:129-137.

Vinardell J-M, Acosta-Jurado S, Zehner S, Göttfert M, Becker A, Baena I, Blom J, Crespo-Rivas JC, Goesmann A, Jaenicke S, Krol E, McIntosh M, Margaret I, Pérez-Montaño F, Schneiker-Bekel S, Serrania J, Szczepanowski R, Buendía AM, Lloret J, Bonilla I, Pühler A, Ruiz-Sainz J-E, Weidner S (2015). The Sinorhizobium fredii HH103 genome: a comparative analysis with S. fredii strains differing in their symbiotic behaviour with soybean. Mol Plant Microbe Interact: 28:811-824.

Schlüter J-P, Czuppon P, Schauer O, Pfaffelhuber P, McIntosh M, Becker A (2015) Classification of phenotypic subpopulations in isogenic bacterial cultures by triple promoter probing at single cell level. J Biotechnol: 198:3-14

Charoenpanich P, Soto MJ, Becker A, McIntosh M (2015) Quorum sensing restrains growth and is rapidly inactivated during domestication of Sinorhizobium meliloti. Environ Microbiol Rep. 7: 373-382.

2014

Krol E, Becker A (2014) Rhizobial homologs of long-chain fatty acid transporter FadL facilitate perception of long chain N-acyl-homoserine lactone signals. Proc Natl Acad Sci USA: 111 10702-10707.

Dugé de Bernonville T, Noël LD, SanCristobal M, Danoun S, Becker A, Soreau P, Arlat M, Lauber E (2014) Transcriptional reprogramming and phenotypical changes associated with growth of Xanthomonas campestris pv. campestris in cabbage xylem sap. Microbiology : Microbiology , Vol. 89 (3) 527-541.

Torres-Quesada O, Reinkensmeier J, Schlüter J-P, Robledo M, Peregrina A, Giegerich R, Toro N, Becker A, Jiménez-Zurdo JI (2014) Genome-wide profiling of Hfq-binding RNAs uncovers extensive post-transcriptional rewiring of major stress response and symbiotic regulons in Sinorhizobium meliloti. RNA Biol. Vol. 11 (5) 563-579.

Baumgardt K, Charoenpanich P, McIntosh M, Schikora A, Stein E, Thalmann S, Kogel K-H, Klug G, Becker A, Evguenieva-Hackenberg E (2014) RNase E affects the expression of the acyl-homoserine lactone synthase gene sinI in Sinorhizobium meliloti. J Bacteriol April 2014, Vol. 196 (7) 1435-1447.

2013

McIntosh M, Czuppon P, Best K, Becker A, Pfaffelhuber P (2013) Modeling quorum sensing in Sinorhizobium meliloti. IJBB 2(1):59-74.

Schmidtke C, Abendroth U, Brock J, Serrania J, Becker A, Bonas U (2013) Small RNA sX13: a multifaceted virulence factor of the plant pathogen Xanthomonas. PLOS Pathogens: 9:  e1003626

Pini F, Frage B, Ferri L, De Nisco NJ, Moapatra S, Taddei L, Firoavanti A, Dewitte F, Galardini M, Brilli M, Villeret V, Bazzicalupo M, Mengoni A, Walker GC, Becker A, Biondi EG (2013) The DivJ, CbrA and PleC system controls DivK phosphorylation and symbiosis in Sinorhizobium meliloti. Mol Microbiol 90: 54–71

Carvalhais LC, Dennis PG, Fan B, Fedoseyenko D, Kierul K, Becker A, von Wiren N, Borriss R(2013) Linking plant nutritional status to plant-microbe interactions. PLoS ONE 8(7): e68555

Charoenpanich P, Meyer S, Becker A, McIntosh M (2013) Temporal expression program of quorum sensing-based transcription regulation in Sinorhizobium meliloti. J Bacteriol 195: 3224-3236

Schlüter JP, Reinkensmeier J, Barnett MJ, Lang C, Krol E, Giegerich R, Long SR, Becker A (2013) Global mapping of transcription start sites and promoter motifs in the symbiotic alpha-proteobacterium Sinorhizobium meliloti 1021. BMC Genomics 14: 156.

Weidner S, Baumgarth B, Göttfert M, Jaenicke S, Pühler A, Schneiker-Bekel S, Serrania J, Szczepanowski R, Becker A (2013) Genome Sequence of Sinorhizobium meliloti Rm41. GenomeA 1: e00013-12.

 

2012

Sobrero P, Schlüter JP, Lanner U, Schlosser A, Becker A, Valverde C (2012) Quantitative proteomic analysis of the Hfq-regulon in Sinorhizobium meliloti 2011. PLoS One: 7(10): e48494.

Shidore T, Dinse T, Öhrlein J, Becker A, Reinhold-Hurek B (2012) Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol: 14: 2775-2787

Fan B, Carvalhais LC, Becker A, Fedoseyenko D, von Wirén N, Borriss R (2012) Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates. BMC Microbiol 12: 116.

Flügel M, Becker A, Gartemann K-H, Eichenlaub R (2012) Analysis of the interaction of Clavibacter michiganensis subsp. michiganensis with its host plant tomato by genome-wide expression profiling. J Biotechnol 160: 42-54.

Agrawal-Singh S, Isken F, Agelopoulos K, Klein HU, Thoennissen NH, Koehler G, Hascher A, Bäumer N, Berdel WE, Thiede C, Ehninger G, Becker A, Schlenke P, Wang Y, McClelland M, Krug U, Koschmieder S, Büchner T, Yu DY, Singh SV, Hansen K, Serve H, Dugas M, Müller-Tidow C (2012) Genome-wide analysis of histone H3 acetylation patterns in AML identifies PRDX2 as an epigenetically silenced tumor suppressor gene. Blood 119: 2346-2357.

Weidner S, Becker A, Bonilla I, Jaenicke S, Lloret J, Margaret I, Pühler A, Ruiz-Sainz JE, Schneiker-Bekel S, Szczepanowski R, Vinardell JM, Zehner S, Göttfert M (2012) Genome sequence of the soybean symbiont Sinorhizobium fredii HH103. J Bacteriol 194: 1617.

Hauberg-Lotte L, Scharf C, Völker U, Becker A, Reinhold-Hurek B (2012) Characterization of population-density regulated gene and protein expression of the grass endophyte Azoarcus sp. BH72. PLoS ONE 7(1): e30421.

 

2011

Reinkensmeier J, Schlüter J-P, Giegerich R, Becker A (2011) Conservation and occurrence of trans-encoded sRNAs in the Rhizobiales. Genes2(4): 925-956.

Schatschneider S, Vorhölter FJ, Rückert C, Becker A, Eisenreich W, Pühler A, Niehaus K (2011) Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C isotopologue profiling. Mol Genet Genomics 286:247-259.

Mayer L, Tondo Vendruscolo C, Padilha da Silva W, Vorhölter F-J, Becker A, Pühler A (2011) Insights into the genome of the phytopathogen Xanthomonas arboricola pv. pruni 109 by comparative genomic hybridization. J Biotechnol 155: 40-49.

Krol E, Becker A (2011) ppGpp in Sinorhizobium meliloti: biosynthesis in response to sudden nutritional downshifts and modulation of the transcriptome. Mol Microbiol 81: 1233–1254.

Joseph B, Schwarz RF, Linke B, Blom J, Becker A, Claus H, Goesmann A, Frosch M, Müller T, Vogel U, Schoen C (2011) Virulence Evolution of the Human Pathogen Neisseria meningitidis by Recombination in the Core and Accessory Genome. PLoS ONE 6(4): e18441.

Sabirova J, Becker A, Lünsdorf H, Nicaud L-M, Timmis KN, Golyshin PN (2011) Trancsriptional profiling of the marine oil-1 degrading bacterium Alcanivorax borkumensis during growth on n-alkanes. FEMS Microbiol Lett 319: 160-168.

Venkatesh AG, Herth S, Becker A, Reiss G (2011) Orientation-defined alignment and immobilization of DNA between specific surfaces. Nanotechnology 22: 145301.

Tickenbrock L, Klein H-U, Trento C, Hascher A, Göllner S, Bäumer N, Kuss R, Agrawal S, Bug G, Serve H, Thiede C, Ehninger G, zur Stadt U, McClelland M, Wang Y, Becker A, Koschmieder S, Berdel WE, Dugas M, Müller-Tidow C (2011) Increased HDAC1 deposition at hematopoietic promoters in AML and its association with patient survival. Leuk Res 35: 620-625.

Martin CW, Tauchen A, Becker A, Nattkemper TW (2011) A Normalized Tree Index for identification of correlated clinical parameters in microarray experiments. Journal of Biomedical Informatics 4: 2.

Krol E, Blom J, Winnebald J, Berhörster A, Barnett MJ, Capela D, Goesmann A, Baumbach J, Becker A (2011) RhizoRegNet – a database of rhizobial transcription factors and regulatory networks. J Biotechnol 140(1-2): 45-50.

Borriss R, Chen XH, Rueckert C, Blom J, Becker A, Baumgarth B, Fan B, Pukall R, Schumann P, Spröer C, Junge H, Vater J, Pühler A, Klenk H-P (2011) Relationship of Bacillus amyloliquefaciens clades associated 1 with strains DSM 7T and FZB42: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discriminating complete genome sequences. Int J Syst Evol Microbiol 61:1786-1801. PMID:20817842

 

2010

Bordes P, Lavatine L, Phok K, Barriot R, Boulanger A, Castanié-Cornet MP, Becker A, Arlat M, Gutierrez C (2010) Insights into the extracytoplasmic stress response of Xanthomonas campestris pv. campestris : Role and regulation of sE-dependent activity. J Bacteriol 193: 246-264.

Joseph B, Schneiker-Bekel S, Schramm-Glück A, Blom J, Claus H, Linke B, Schwarz R, Becker A, Goesmann A, Frosch M, Schoen C (2010) Comparative genome biology of a serogroup B carriage and disease strain supports a polygenic nature of meningococcal virulence. J Bacteriol 192: 5363–5377.

Greif D, Pobigaylo N, Frage B, Becker A, Regtmeier J, Anselmetti D (2010) Space- and time-resolved protein dynamics in single bacterial cells observed on a microfluidics chip. J Biotechnol 149: 280-288.

Müller-Tidow C, Klein H-U, Hascher A, Isken F, Tickenbrock L, Thoennissen N, Agrawal-Singh S, Tschanter P, Disselhoff C, Wang Y, Becker A, Thiede C, Ehninger G, zur Stadt U, Koschmieder S, Seidl M, Müller FU, Schmitz W, Schlenke P, McClelland M, Berdel WE, Dugas D, Serve H (2010) Profiling of histone H3 lysine 9 trimethylation levels predicts transcription factor activity and survival in acute myeloid leukemia. Blood 116: 3564-3571.

Schlüter J-P, Reinkensmeier J, Daschkey S, Evguenieva-Hackenberg E, Janssen S, Jänicke S, Becker JD, Giegerich R, Becker A (2010) A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing a-proteobacterium Sinorhizobium meliloti. BMC Genomics 11: 245.

 

2009

McIntosh M, Meyer S, Becker A (2009) Novel Sinorhizobium meliloti quorum sensing positive and negative regulatory feedback mechanisms respond to phosphate availability. Mol Microbiol 74: 1238-1256.

Voss B, Hölscher M, Baumgarth B, Kalbfleisch A, Kaya C, Hess WR, Becker A, Evguenieva-Hackenberg E (2009) Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti. Biochem Biophys Res Comm: 390, 331-336.

Jofré E, Becker A (2009) In Sinorhizobium meliloti production of succinoglycan polymer is affected by SMb21506 and requires the N-terminal domain of ExoP. Mol Plant-Microbe Interact 22: 1656-1668.

Wollschläger K, Gaus K, Körnig A, Eckel R, Wilking SD, McIntosh M, Majer Z, Becker A, Ros R, Anselmetti D, Sewald S (2009) Single molecule experiments to elucidate the minimal requirement for DNA recognition by transcription factor epitopes. Small 5: 484-495.

Becker A, Barnett MJ, Capela D, Dondrup M, Kamp P-B, Krol E, Linke B, Rüberg S, Runte K, Schroeder BK, Weidner S, Yurgel S, Batut J, Long SR, Pühler A, Finan TM, Goesmann A (2009) A portal for rhizobial genomes: RhizoGATE integrates a S. meliloti genome annotation update with postgenome data. J Biotechnol 140: 45-50.

 

2008

Valverde C, Livny J, Schlüter J-P, Reinkensmeier J, Becker A, Parisi G (2008) Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011. BMC Genomics 9: 416.

Bahlawane C, McIntosh M, Krol E, Becker A (2008) Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility. Mol Plant-Microbe Interact 21: 1498-1509.

McIntosh M, Krol E, Becker A (2008) Competitive and co-operative effects in quorum sensing regulated galactoglucan biosynthesis in Sinorhizobium meliloti. J Bacteriol 190: 5308–5317.

Stiens M, Becker A, Bekel T, Gödde V, Goesmann A, Niehaus K, Schneiker-Bekel S, Selbitschka W, Weidner S, Schlüter A, Pühler A (2008) Comparative genomic analysis and ultrafast pyrosequencing revealed remarkable differences between the Sinorhizobium meliloti genomes of the model strain Rm1021 and the field isolate SM11. J Biotechnol 136(1-2): 31-37.

Serrania J, Vorhölter F-J, Niehaus K, Pühler A, Becker A (2008) Identification of Xanthomonas campestris pv. campestris galactose utilization genes from transcriptome data. J Biotechnol 135: 309-317.

Cosme AM, Becker A, Santos MR, Sharypova LA, Santos PM, Moreira LM (2008) The outer membrane protein TolC from Sinorhizobium meliloti affects protein secretion, polysaccharide biosynthesis, antimicrobial resistance and symbiosis. Mol Plant-Microbe Interact 21: 947-957.

Bahlawane C, Baumgarth B, Serrania J, Rüberg S, Becker A (2008) Fine-tuning of galactoglucan biosynthesis in Sinorhizobium meliloti by differential ExpG- (WggR‑), PhoB-, and MucR-dependent regulation of two promoters. J Bacteriol 190: 3456-3466.

Nodop A, Pietsch D, Becker A, Pistorius EK, Forchhammer K, Michel K-P (2008) DNA microarray analysis of Synechococcus elongatus PCC 7942 wild type, an IdiB-free mutant, and an idiC-merodiploidmutant grown in the presence or absence of iron. Plant Physiol 147:747-763.

Schriek S, Aguirre-von-Wobeser E, Nodop A, Becker A, Ibelings BW,  Bok J, Staiger D, Matthijs HC, Pistorius EK, Michel KP (2008) Transcript-profiling indicates that the absence of PsbO affects the coordination of C and N metabolism in Synechocystis sp. PCC 6803. Physiol Plant 133: 525-543.

Vijayendran C, Barsch A, Niehaus K, Becker A, Flaschel E (2008) Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling. Genome Biology 9:R72,

Vorhölter FJ, Schneiker S, Goesmann A, Krause L, Bekel T, Kaiser O, Linke B, Patschkowski T, Rückert C, Schmid J, Sidhu VK, Sieber V, Tauch A, Watt SA, Weisshaar B, Becker A, Niehaus K, Pühler A (2008) The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis. J Biotechnol 134: 33-45.

Pobigaylo N, Szymczak S, Nattkemper TW, Becker A (2008) Identification of genes relevant to symbiosis and competitiveness in Sinorhizobium meliloti using signature-tagged mutants. Mol Plant-Microbe Interact 21: 219-231.

 

2007

Nyamsuren O, Firnhaber C, Hohnjec N, Becker A, Küster H, Krajinski F (2007) Suppression of the pathogen-inducible Medicago truncatula putative protease-inhibitor MtTi2 does not influence root infection by Aphanomyces euteiches but results in transcriptional changes from wild type roots. Plant Sci 173: 84-95.

Bartels FW, McIntosh M, Metzendorf C, Plattner P, Sewald N, Anselmetti D, Ros R, Becker A (2007) Effector-stimulated single molecule protein-DNA interactions of a quorum-sensing system  in Sinorhizobium meliloti. Biophys J 92: 4391-4400.

Perrine-Walker FM, Gartner E, Hocart CH, Becker A, Rolfe BG (2007) Rhizobium-initiated rice growth inhibition caused by nitric oxide accumulation. Mol Plant-Microbe Interact 20: 283-292.

 

2006

Yao Z, Tian Z, Dai X, Becker A, Li J, Yan H, Xiao Y, Zhu J, Yu G, RübergS, Wang Y,  Zou H (2006) Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes. Chinese Science Bulletin 51:2748-2754.

Tian Z, Zou H, Li J, Zhang Y, Liu Y, Yu G, Zhu J, Rüberg S, Becker A, Wang Y (2006) Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background. Chinese Science Bulletin 51: 2079-2086.

Tian Z, Mao X, Su W, Li J, Becker A, Wang Y-P (2006) Exogenous cAMP upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021. Chinese Science Bulletin 51: 1982-1985.

Sharypova LA, Chataigne G, Fraysse N, Becker A, Poinsot V (2006) Overproduction and increased molecular weight account for the symbiotic activity of the rkpZ-modified K polysaccharide from Sinorhizobium meliloti Rm1021. Glycobiology 16: 1181-93.

Domínguez-Ferreras A, Pérez-Arnedo R, Becker A, Olivares J, Soto MJ, Sanjuán J (2006) Transcriptome profiling reveals the importance of plasmid pSymB for osmoadaptation of Sinorhizobium meliloti. J Bacteriol 188: 7617-7625.

Brune I, Becker A, Paarmann D, Albersmeier A, Kalinowski J, Pühler A, Tauch A (2006) Under the influence of the active deodorant ingredient 4-hydroxy-3-methoxybenzyl alcohol, the skin bacterium Corynebacterium jeikeium moderately responds with differential gene expression. J Biotechnol 127: 21-33.

Buitink J, Guisle I, Le Meur N, Ly Vu B, Wuillème S, Leger J, Becker A, Küster H, Leprince O (2006) Transcriptome profiling uncovers metabolic and regulatory processes occuring during the transition from dessication-sensitive to dessication-tolerant stages in Medicago truncatula seeds. Plant J 47: 735-750.

Pobigaylo N, Wetter D, Szymczak S, Schiller U, Kurtz S, Meyer F, Nattkemper TW, Becker A (2006) Construction of a large sequence signature-tagged miniTn5 transposon library and its application to mutagenesis of Sinorhizobium meliloti. Appl Environ Microbiol 72: 4329-4337.

Plattner HP, Metzendorf C, McIntosh M, Bartels FW, Anselmetti D, Ross R, Becker A, Sewald N (2006) Synthesis of modified N-acylhomoserinelactones. J Peptide Science 12: 207.

 

2005

Giuntini E, Mengoni A, De Filippo C, Cavalieri D, Aubin-Horth N, Landry CR, Becker A, Bazzicalupo M (2005) Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by  comparative genomic analysis of Sinorhizobium  meliloti natural strains. BMC Genomics 6: 158.

Luo L, Yao S-Y, Becker A, Rüberg S, Yu Q-Q, Zhu J-B, Cheng HP (2005) Identification of two new Sinorhizobium meliloti LysR-like transcriptional regulators required in nodulation. J Bacteriol 187: 4562-4572.

Eckel R, Wilking SD, Becker A, Sewald N, Ros R, Anselmetti D (2005) Single molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides (p NA). Angew Chem Int Ed 44: 3921-3924.

Hohnjec N, Vieweg MF, Pühler A, Becker A, Küster H (2005) Overlaps in the transcriptional profiles of Medicago truncatula roots inoculated with two different Glomus spec. fungi provide insights into the genetic program activated during arbuscular mycorrhiza. Plant Physiol 137: 1283-1301.

Baumgarth B, Bartels FW, Rüberg S, Anselmetti A, Becker A, Ros R (2005) Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA. Microbiology 151: 259-268.

Moreira LM, Hoffmann K, Albano H, Becker A, Niehaus K, Sá-Correia I (2005) Functional analysis of the gellan genes gelC and gelE from Sphingomonas elodea ATCC 31461 encoding two separate polypeptides homologous to the activator and to the kinase domains of phosphotyrosine autokinases.  J Mol Microbiol Biotechnol 8: 43-57.

Onishchuk OP, Sharypova LA, Kurchak ON, Becker A, Simarov BV (2005) Identification of Sinorhizobium meliloti genes influencing synthesis of surface polysaccharides and competitiveness. Genetika 41:1617-1623.

 

2004

Brückl H, Brzeska M, Brinkmann D, Schotter J, Reiss G, Schepper W, Kamp PB, Becker A (2004) Magnetoresistive logic and biochip. J Magn Magn Mater 282: 219-224.

Puskás L, Nagy Z, Kelemen JZ, Rüberg S, Bodogai M, Becker A, Dusha I (2004) Wide-range transcriptional modulating effect of ntrR under microaerobiosis in Sinorhizobium meliloti. Mol Gen Genomics 272: 275-289.

El Yahyaoui F, Küster H, Ben Amor B, Hohnjec N, Pühler A, Becker A, Gouzy J, Vernié T, Gough C, Niebel A, Godiard L, Gamas P (2004) Expression profiling in Medicago truncatula identifies more than 900 genes differentially expressed during nodulation, including many potential regulators of the symbiotic program. Plant Physiol 136: 3159-3176.

Yao S-Y, Luo L, Har KJ, Becker A, Rüberg S, Yu G-Q, Zhu JB, Cheng H-P (2004) The Sinorhizobium meliloti ExoRand ExoS proteins regulate both succinoglycan and flagella production. J Bacteriol 186: 6042-6049.

Krol E, Becker A (2004) Global transcriptional analysis of phosphate stress responses in Sinorhizobium meliloti strains 1021 and 2011. Mol Gen Genomics 272: 1-17.

Grunwald U, Nyamsuren O, Tamasloukht M, Lapopin L, Becker A, Mann P, Gianinazzi-Pearson V, Krajinski F, Franken P (2004) Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. Plant Mol Biol 55: 553-566.

Ros R, Eckel R, Bartels F, Sischka A, Baumgarth B, Wilking SD, Pühler A, Sewald N, Becker A, Anselmetti D (2004) Single molecule force spectroscopy on ligand-DNA complexes: From molecular binding mechanisms to biosensor applications. J Biotechnol 112: 5-12.

Brzeska M, Panhorst M, Kamp PB, Schotter J, Reiss G, Pühler A, Becker A, Brückl H (2004) Detection and manipulation of biomolecules by magnetic carriers. J Biotechnol 112: 25-33.

Hoang HH, Becker A, González JE (2004) The LuxR homolog ExpR, in combination with the Sin quorum sensing system, plays a central role in Sinorhizobium meliloti gene expression. J Bacteriol 186: 5460-5472.

Quester I, Becker A (2004) Four promoters subject to regulation by ExoR and PhoB direct transcription of the Sinorhizobium meliloti exoYFQ operon involved in biosynthesis of succinoglycan. J Mol Microbiol Biotechnol 7: 115-132.

Chao T-C, Becker A, Buhrmester J,  Pühler A, Weidner S  (2004) The Sinorhizobium meliloti fur gene regulates in dependence of Mn(II) the transcription of the sitABCD operon encoding a metal type transporter. J Bacteriol 186: 3609-3620.

Becker A, Bergès H, Krol E, Bruand C, Rüberg S, Capela D, Lauber E, Meilhoc E, Ampe A, de Bruijn FJ, Fourment J, Francez-Charlot A, Kahn D, Küster H, Liebe C, Pühler A, Weidner S, Batut J (2004) Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Mol Plant-Microbe Interact 17: 292-303.

Küster H, Hohnjec N, Krajinski F, El Yahyaoui F, Manthey K, Gouzy J, Dondrup M, Meyer F, Kalinowski J, Brechenmacher L, van Tuinen D, Gianinazzi-Pearson V, Pühler A, Gamas P, Becker A (2004) Construction and validation of comprehensive cDNA-based macro- and microarrays to explore root nodule endosymbioses in the model legume Medicago truncatula. J Biotechnol 108: 95-113.

Schotter J, Kamp PB, Becker A, Pühler A, Reiss, G, Brückl H (2004) Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA-detection. Biosensors and Bioelectronics 19: 1149-1156.

 

2003

Bartels F, Baumgarth B, Anselmetti D, Ros R, Becker A (2003) Specific binding of the regulatory protein ExpG to promoter regions of the galactoglucan biosynthesis gene cluster of Sinorhizobium meliloti - A combined molecular biology and force spectroscopy investigation. J Struct Biol 143: 145-152.

Rüberg S, Tian ZX, Krol E, Linke B, Meyer F, Wang Y, Pühler A, Weidner S, Becker A (2003) Construction and validation of a DNA microarray for genome-wide expression profiling in Sinorhizobium meliloti. J Biotechnol 106: 255-268.

Hüser AT, Becker A, Brune I, Dondrup M, Kalinowski J, Plassmeier J, Pühler A, Wiegräbe I, Tauch A (2003) Development of a Corynebacterium glutamicum DNA microarray and validation by genome-wide expression profiling during growth with propionate as carbon source. J Biotechnol 106: 269-286.

Sharypova LA, Niehaus K, Scheidle H, Holst O, Becker A (2003) Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express smooth LPS. J Biol Chem 278: 12946-12954.

Campbell G, Scheidle H, Sharypova LA, Jones K, Niehaus K, Becker A, and Walker GC (2003) Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants. J Bacteriol 185: 3853-3862.

 

2002

Schotter J, Kamp PB, Becker A, Pühler A, Brinkmann D, Schepper W, Brückl H, Reiss G (2002) A Biochip based on magnetoresistive sensors. IEEE Trans Mag 38: 3365-3367.

 

2001

Niemeyer D, Becker A (2001) The molecular weight distribution of succinoglycan produced by Sinorhizobium meliloti is influenced by specific phosphorylation and ATPase activity of the cytoplasmic domain of the ExoP protein. J Bacteriol 183: 5163-5170.

Schröter K, Flaschel E, Pühler A, Becker A (2001) Xanthomonas campestris pv. campestris secretes the endoglucanases ENGXCA and ENGXCB: construction of an endoglucanase deficient mutant for industrial xanthan production. Appl Microbiol Biotech 55: 727-733.

Galibert F, Finan TM, Long SR,Pühler A, Abola P, Ampe F, Barloy-Hubler F, Barnett MJ, Becker A, Boistard P, Bothe G, Boutry M, Bowser L, Buhrmester J, Cadieu E, Capela D, Chain P, Cowie A, Davis RW, Dréano S, Federspiel NA, Fisher RF, Gloux S, Godrie T, Goffeau A, Golding B, Gouzy J, Gurjal M, Hernandez-Lucas I, Hong A, Huizar L, Hyman RW, Jones T, Kahn D, Kahn ML, Kalman S, Keating D, Kiss E, Komp C, Lelaure V, Masuy D, Palm C, Peck MC, Pohl T, Portetelle D, Purnelle B, Ramsperger U, Surzycki R, Thébault P, Vandenbol M, Vorhölter F-J, Weidner S, Wells DH, Wong K, Yeh K-C, Batut J (2001) The composite genome of the legume symbiont Sinorhizobium meliloti. Science 293: 668-672.

Capela D, Barloy-Hubler F, Gouzy J, Bothe G, Ampe F, Batut J, Boistard P, Becker A, Boutry M, Cadieu E, Dréano S, Gloux S, Godire T, Goffeau A, Kahn D, Lelaure V, Masuy D, Pohl T, Portelle D, Pühler A, Purnelle B, Ramsperger U, Thébault P, Vandenbol M, Weidner S, Galibert F (2001) Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti. Proc Nat Acad Sci USA 98: 9877-9882.

Finan TM, Weidner S, Chain P, Becker A, Wong K, Cowie A, Buhrmester J, Vorhölter F-J, Golding B, Pühler A (2001) The complete sequence of the Sinorhizobium meliloti pSymB megaplasmid. Proc Nat Acad Sci USA 98: 9889-9894.

 

2000

Moreira LM, Becker JD, Pühler A, Becker A (2000) The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. Microbiology 146: 2237-2248.

 

1999

Katzen F, Becker A, Ielmini MV, Oddo CG, Ielpi L (1999) New mobilizable vectors suitable for gene replacement in Gram-negative bacteria and its use to map the 3' end of the Xanthomonas campestris pv. campestris gum operon. Appl Environ Microbiol 65: 278-282.

Rüberg S, Pühler A, Becker A (1999) Biosynthesis of the exopolysaccharide galactoglucan in Sinorhizobium meliloti is subject to a complex control by the phosphate-dependent regulator PhoB and the proteins ExpG and MucR. Microbiology 145: 603-611.

Sharypova LA , Yurgel SN , Keller M, Simarov BV, Pühler A, Becker A (1999) The eff-482 locus of Sinorhizobium meliloti CXM1-105 that influences symbiotic effectiveness consists of three genes encoding an endoglycanase, a transcriptional regulator and an adenylate cyclase. Mol Gen Genet 261: 1032-1044.

1998

Katzen F, Ferreiro DU, Oddo CG, Ielmini V, Becker A, Pühler A, Ielpi L (1998) Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence. J Bacteriol 180: 1607-1617.

Becker A, Pühler A (1998) Specific amino acid substitutions in the proline-rich motif of the Rhizobium meliloti ExoP protein result in enhanced production of low-molecular-weight succinoglycan at the expense of high-molecular-weight succinoglycan. J Bacteriol 180: 395-399.

Bertram-Drogatz PA, Quester I, Becker A, Pühler A (1998) The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. Mol Gen Genet 257: 433-441.

Jacq C, Alt-Mörbe J, Andre B, Arnold W, Bahr A, Ballesta JPG, Bargues M, Baron L, Becker A, et al. (1997) The nucleotide sequence of Saccharomyces cerevisiae chromosome IV. Nature 387 (suppl.): 75‑78.

 

1997

Bertram-Drogatz PA, Rüberg S, Becker A, Pühler A (1997) The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol Gen Genet 254: 529-538.

Becker A, Rüberg S, Küster H, Roxlau AA, Keller M, Ivashina T, Cheng H-P, Walker GC, Pühler A (1997) The 32-kb exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products. J Bacteriol 179: 1375-1384.

 

1996

Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L (1996) Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing the biosynthesis of the xanthan polysaccharide. J Bacteriol 178: 4313-4318.

 

1995

Becker A, Küster H, Niehaus K, Pühler A (1995) Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster: identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesis. Mol Gen Genet 249: 487-497.

Becker A, Schmidt M, Jäger W, Pühler A (1995) New gentamicin resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene 162: 37-39.

Becker A, Niehaus K, Pühler A (1995) Low molecular weight succinoglycan is predominantly produced by Rhizobium meliloti strains carrying a mutated ExoP protein characterized by a periplasmic N-terminal and a missing C-terminal domain. Mol Microbiol 16: 191-203.

 

1993

Becker A, Kleickmann A, Küster H, Keller M, Arnold W, Pühler A (1993) Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT and exoI involved in exopolysaccharide biosynthesis and nodule invasion: exoU and exoW probably encode glucosyltransferases. Mol Plant-Microbe Interact 6: 735-744.

Becker A, Kleickmann A, Keller M, Arnold W, Pühler A (1993) Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment. Mol Gen Genet 241: 367-379.

Becker A, Kleickmann A, Arnold W, Pühler A (1993) Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-b-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet 238: 145-154.

 

Reviews

2017

Becker A (2017) Classic Spotlight: Bacteroids - views of an enigmatic bacterial state in root nodule symbiosis through the centuries. J Bacteriol 199:e00741-16

2016

Becker A (2016) Classic Spotlight: Hfq, from a Specific Host Factor for Phage Replication to a Global Player in Riboregulation. J Bacteriol 198:2279-2280

2015

Becker A (2015) Challenges and perspectives in combinatorial assembly of novel exopolysaccharide biosynthesis pathways. In: Schmid J (ed.).  Front. Microbiol. - Microbiotechnology, Ecotoxicology and Bioremediation.ISSN: 1664-302X, Manuscript ID: 144903

2014

Anke Becker, Aaron Overlöper, Jan-Philip Schlüter, Jan Reinkensmeier, Marta Robledo, Robert Giegerich, Franz Narberhaus, Elena Evguenieva-Hackenberg, Franz Narberhaus (2014) Riboregulation in plant-associated a-proteobacteria. RNA Biology 11 (5), 550-749.

2013

Becker A: Construction of signature-tagged mutant libraries and its application to plant-symbiotic bacteria. In: de Bruijn F (ed.). Molecular Microbial Ecology of the Rhizosphere. Chapter 86. Wiley/Blackwell. ISBN 978-1- 1182-9617- 2

Jiménez-Zurdo JI, Valverde C, Becker A: Insights into the non-coding RNome of nitrogen-fixing endosymbiotic alpha-proteobacteria. Mol Plant Microbe Interact 26: 160-167. PMID: 22991999

2012

 

2011

Margaret I, Becker A, Blom J, Bonilla I, Goesmann A, Göttfert M, Lloret J, Mittard-Runte V, Pühler A, Rückert C, Ruiz-Sainz JE, Vinardell JM, Weidner A (2011) Symbiotic properties and first analyses of the genomic sequence of the fast growing model strain Sinorhizobium fredii HH103 nodulating soybean. J Biotechnol 155: 11-19.

 

2009

Krol E, Becker A (2009) Surface polysaccharides as fitness factors of nitrogen-fixing plant-associated bacteria. In: Ullrich M (ed.). Bacterial Polysaccharides: Current Innovations and Future Trends. Horizon Press. pp. 189-211.

Becker A (2009) Functional Genomics of Rhizobia. In: Pawlowski K (ed.). Prokaryotic Symbionts in Plants (Microbiology Monographs, Volume 8). Springer, Heidelberg, Germany. pp. 71-100.

 

2008

Anselmetti D, Bartels FW, Becker A, Decker B, Eckel R, McIntosh M, Mattay J, Plattner P, Ros R, Schäfer C, Sewald N (2008) Reverse Engineering of an Affinity-Switchable Molecular Interaction Characterized by AFM Single Molecule Force Spectroscopy. Langmuir 24: 1365-1370.

Küster H, Becker A (2008) Microarrays as tools to decipher transcriptomes in symbiotic interactions. In: Kahl G, Meksem K (eds.). The Handbook of Plant Functional Genomics. Wiley-VCH. pp. 137-162.

Becker A, Vorhölter F-J (2008) Xanthan biosynthesis by Xanthomonas bacteria – an overview of the current biochemical and genomic data. In: Rehm BHA (ed.). Microbial production of biopolymers and polymer precursors: Applications and perspectives. Caister Academic Press, Norfolk, UK. pp. 1-11.

 

2007

Martini J, Hellmich W, Greif D, Becker A, Merkle T, Ros R, Ros A, Tönsing K, Anselmetti D (2007) Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis. Subcell Biochem 43: 301-321.

Küster H, Becker A, Firnhaber C, Hohnjec N, Manthey K, Perlick AM, Bekel T, Dondrup M, Henckel K, Goesmann A, Meyer F, Wipf D, Requena N, Hildebrandt U, Hampp R, Nehls U, Krajinski F, Franken P, Pühler A (2007) Development of bioinformatic tools to support EST-sequencing, in silico- and microarray-based transcriptome profiling in mycorrhizal symbioses. Phytochemistry 68: 19-32.

 

2006

Sewald N, Wilking SD, Eckel R, Albu S, Wollschlager K, Gaus K, Becker A, Bartels FW, Ros R, Anselmetti D (2006) Probing DNA-peptide interaction forces at the single-molecule level. J Pept Sci 12(12): 836-842.

 

2005

Becker A, Fraysse N, Sharypova LA (2005) Recent advances in studies on structure and symbiosis-related function of rhizobial K-antigens and lipopolysaccharides. Mol Plant-Microbe Interact 18: 899-905.

Reiss G, Brückl H, Huetten A, Schotter J, Brzeska M, Panhorst M, Sudfeld D, Becker A, Kamp PB, Pühler A, Wojczykowski K, Jutzi P (2005) Magnetoresistive Sensors and Magnetic Nanoparticles for Biotechnology. J Mater Res  20: 3294.

Brückl H, Panhorst M, Schotter J, Kamp PB, Becker A (2005) Magnetic particles as markers and carriers of biomolecules. IEE Proc Nanobiotechnol 152: 41-46.

Becker A, de Bruijn FJ (2005) Transcriptomics in Sinorhizobium meliloti. In: Palacios R, Newton WE (eds.). Genomes and Genomics of Nitrogen-fixing Organisms. Springer, Dordrecht, The Netherlands. pp. 169-181.

 

2004

Pühler A, Arlat M, Becker A, GöttfertM, Morrissey JP, O’Gara F (2004) What is bacterial genome research telling us about bacteria-plant interactions? Curr Opp Plant Biol 7: 137-147.

Becker A, Pühler A, Küster H: Nahrung aus der Luft - Ein komplexes Zusammenspiel von Regulatoren steuert die Wurzelknöllchensymbiose zwischen Leguminosen und Stickstoff-fixierenden Bodenbakterien. GenomXPress 1.04, pp. 5-9 (2004).

Becker A (2004) Design of microarrays for genome-wide expression profiling. In: Kowalchuk GA, de Bruijn FJ, Head IM, Akkermans ADL, van Elsas JD (eds.). Molecular Microbial Ecology Manual, 2nd edition. Kluwer Academic Publishers, Dordrecht. The Netherlands. pp. 1543-1562.

 

2002

Becker A, Rüberg S, Baumgarth B, Bertram-Drogatz PA, Quester I, Pühler A (2002) Regulation of succinoglycan and galactoglucan biosynthesis in Sinorhizobium meliloti. J Mol Microbiol Biotech 4: 187-190.

 

2001

Weidner S, Becker A, Pühler A (2001) Mikrobielle Genomforschung in Deutschland am Beispiel des Megaplasmids pSymb aus Sinorhizobium meliloti. BioSpektrum 3.01, 7. Jahrgang: 226-228.

 

2000

Becker A, Niehaus K, Pühler A (2000) The role of rhizobial extracellular polysaccharides (EPS) in the Sinorhizobium meliloti – alfalfa symbiosis. In: Triplett E (ed.). Nitrogen Fixation in Prokaryotes: Molecular and Cellular Biology. Horizon Scientific Press. pp. 433-447.

 

1998

Becker A, Katzen F, Pühler A, Ielpi L (1998) Xanthan gum biosynthesis and application: a biochemical-genetic perspective. Appl Microbiol Biotechnol 50: 145-152.

Becker A, Rüberg S, Quester I, Bertram-Drogatz PA, Schlößer H, Moreira LM, Sharypova L, Pühler A (1998) Genetics of succinoglycan and galactoglucan biosynthesis in Sinorhizobium meliloti. In: Steinbüchel A (ed.). Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers. Wiley-VCH-Verlag, Weinheim, Germany. pp. 113-121.

Niehaus K, Becker A (1998) The role of microbial surface polysaccharides in the Rhizobium-legume interaction. In: Biswas BB, Das HK (eds.). Subcellular Biochemistry, Vol. 29: Plant-Microbe Interactions. Plenum Press, New York, USA. pp. 73-115.

Becker A, Pühler A (1998) Production of exopolysaccharides. In: Spaink HP, Kondorosi A, Hooykaas PJJ (eds.). The Rhizobiaceae. Molecular Biology of Model Plant-Associated Bacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands. pp. 97-118.

Katzen F, Ielmini V, Oddo CG, Ferreiro DU, Becker A, Pühler A, Ielpi L (1998) Biosynthesis of xanthan. In: Steinbüchel A (ed.). Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers. Wiley-VCH-Verlag, Weinheim, Germany. pp. 152-160.

 

1994

Arnold W, Becker A, Keller M, Roxlau A, Pühler A (1994) The role of Rhizobium meliloti surface polysaccharides in the infection of Medicago sativa nodules. Endocytobiosis & Cell Res 10: 17-28.

 

1992

Arnold W, Keller M, Becker A, Roxlau A, Niehaus K, Quandt J, Weng W-M, Pühler A (1992) The surface polysaccharides of Rhizobium meliloti and their role in forming N2-fixing alfalfa nodules. Endocytobiosis & Cell Res 8: 197-229.

 

 

 

SYNMIKRO Young Researchers Groups

Almost all scientific members of SYNMIKRO are actively involved in DFG’s Collaborative Research Centers (Sonderforschungsbereiche), Research Training Groups (Graduiertenkollegs), or other Cooperative Research projects. Alongside performing adventurous experiments, and reporting excellent science, SYNMIKRO substantially promotes potential Young Research Group Leaders by constantly keeping its doors open to welcome and support Young Researchers planning to set up an Independent Research Group.
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